Pee-wee Herman (as Pee-wee Herman)
Yul / Party Coordinator (voice)
Mr. Murgatroyd
Mrs. Rose
Ruby the Crossing Guard
Marvin
Emily
Beverly
Pharmacist
Dan
Dudley
Sylvester
Clovis (as Corey Craig)
Ernie
Construction Worker (as Bobby Ray Shafer)
Construction Worker
Joe Manganiello
Pepper
Freckles
Bella
Police Officer
Gordon the Traveling Salesman
Panicked Female Passenger
Panicked Male Passenger
Clay
Jimmy
Tourist at Snake Farm
Farmer Brown
Linda Brown
Louise Brown
Donna Brown
Diane Brown
Janice Brown
Betty Brown
Pam Brown
Judy Brown
Peggy Brown
Minister
Rene
Brook
Lane
Wanda
Penny King (as Diane Louise Salinger)
Grizzly Bear Daniels
Abe
Ezekiel
New York City Policeman
Guy who bumps into Pee-wee (as Michael R. Pauley)
Pizzeria Employee
DJ
Party Guest
Shelly
Abraham Lincoln
Queen
TV Reporter
TV Cameraman
Police Captain (as Charlie Robinson)
Extra (uncredited)
Jailbird (uncredited)
Man with Toupee (uncredited)
Edgar Tomkins (uncredited)
Diner Patron (uncredited)
Fairville Pedestrian (uncredited)
Construction Worker (uncredited)
Hardware Store Owner (uncredited)
NYC Tourist (uncredited)
Amish Man (uncredited)
Waitress (uncredited)
Guy in Pizza Parlor (uncredited)
Gas Station Attendant (uncredited)
Amish man (uncredited)
UN Russian Ambassador (uncredited)