Ezio Auditore Da Firenze (voice)
Lucy Stillman (voice)
Desmond Miles (voice)
Mario Auditore (voice)
Leonardo da Vinci (voice)
Rodrigo Borgia (voice)
Claudia Auditore da Firenze / Crying Boy (voice)
Federico Auditore da Firenze (voice)
Bartolomeo d'Alviano (voice)
Octavien de Valois (voice)
Lucrezia Borgia (voice)
Shaun Hastings (voice)
Rebecca Crane (voice)
Juan Borgia the Elder (voice)
La Volpe (voice)
Cesare Borgia (voice)
Animus (voice)
Caterina Sforza (voice)
Niccolò Machiavelli / Duccio de Luca - The Da Vinci Disappearance DLC (voice)
Additional Voices (voice)
Cristina Vespucci / Angelica Ceresa / Valencia / Roman Citizen (voice) (as Amber Mullin)
Vinicio / Doctors / Art Merchants / Bankers / Heralds / Tailors / Thieves / Assassin Recruit / Roman Guardsman / Roman Citizen (voice)
Subject 16 / Doctors / Art Merchants / Play Actor / Roman Guardsman / Roman Citizen (voice)
Blacksmiths / Borgia Brutes / Roman Guardsman / Roman Citizen (voice)
Salaì - The Da Vinci Disappearence DLC / Art Merchants (voice)
Maria Auditore da Firenze / Roman Citizen (voice)
Fabio Orsini / Vieri de' Pazzi / Roman Citizen (voice)
Borgia Guard Captain / Heralds / Vigilantes (voice)
Angelina Ceresa / Lia de Russo / Roman Citizen (voice)
Luigi / Heralds / Minstrels / Tailors / Vigilantes / Roman Citizen (voice)
Courtesans / Roman Citizen (voice)
Tommaso di Viterbo / Auguste Oberlin / Vincenzo / Cannon Operator / Blacksmiths / Roman Guardsman / Roman Citizen (voice)
Minerva (voice)
Additional Voices (voice) (as Maurizzio Terrazzano)
Borgia Guard Captain / Play Actor / Mercenaries / Assassin Recruit (voice)
Cannon Engineer / Roman Guardsman / Roman Citizen (voice)
Pantisileia D'Alviano (voice)
Juno / Roman Citizen (voice)
William Miles / Silvestro Sabbatini / Blacksmiths / Minstrels / Tailors / Roman Citizen (voice)
Warren Vidic / Donato Mancini / Minstrels / Tailors / Vigilantes / Roman Citizen (voice)
Additional Voices (voice) (as Richard Dumont)
Micheletto Corella / Additional Voices (voice)
Multiplayer Trainer (voice)
Poison-kill (voice)