Scooby-Doo / Fred Jones / Bikers (voice)
Velma Dinkley (voice)
Daphne Blake (voice) (as Grey DeLisle)
Shaggy Rogers / Shaky Joe (voice)
Faith (voice)
Friz / GPS / Bikers (voice)
Mr. Babbit / Blair / Grad Student #1 / Bikers (voice)
Winsor (voice)
Professor Svankmajer (voice)
Doctor (voice)
Tex / Cop #2 / Grad Student #3 / Bikers (voice)
Mr. Hubley (voice)
Cop #1 / Grad Student #2 / Policeman / Bikers (voice)
Ms. Deitch (voice)
Self (voice)
Aunt Margaret (voice)