Spider-Man / Peter Parker (voice)
Black Cat / Felicia Hardy (voice)
Kingpin / Wilson Fisk (voice)
Kraven the Hunter / Sergei Kravinoff (voice)
Shocker / Herman Schultz (voice)
Carnage / Cletus Kasady (voice)
Green Goblin / Harry Osborn (voice)
Chameleon / Donald Menken (voice)
Electro / Max Dillon (voice) (as Michael A. Shepperd)
Jean DeWolff (voice)
Mary Parker (voice)
Ben Parker (voice)
The Burglar / Dennis Carradine (voice)
Whitney Chang (voice)
J. Jonah Jameson (voice)
Civilian Male / Male Police (voice)
Russian Thug / Russian Mobster (voice)
Civilian Female / Street Speedy Girl (voice)
Civilian Male / Male Police Dispatcher (voice)
Street Thug / Ravencroft Thug (voice)
Task Force Flying / Male Police (voice)
Civilian Male / Task Force Flying (voice)
Russian Thug / Criminal (voice)
Russian Brute / Russian Mobster (voice)
Task Force Brute / Russian Brute (voice)
Civilian Female / Female Police (voice)
Russian Speedy Girl / Female Police (voice)
Street Brute (voice) (as Isaac Singleton Jr.)
Stan Lee (voice)
Hammerhead / Russian Fist (voice)
Electro / Maxwell Dillon (voice)
Green Goblin / Harry Osborn / Kraven the Hunter / Sergei Kravinoff (voice)
Venom / Eddie Brock (voice)
Donald Menken (voice)
Additional Voices (voice) (as Catero Colbert)
Additional Voices (voice)
Times Square Driver (as Stefanie Barry)
Agent Cal
Harry Osborne (voice)
Woman on Plane
Peter Parker / Spider-Man