Booker DeWitt / Rapture Newscaster (voice)
Booker DeWitt / Sander Cohen / Atlas
Elizabeth / Sally (voice)
Daisy Fitzroy (voice) (as Kimberly D. Brooks)
Daisy Fitzroy / Rosalind Lutece
Robert Lutece / Additional Vocies (voice)
Robert Lutece
Rosalind Lutece / Florence Baxter (voice)
Jeremiah Fink / Lonnie (voice)
Andrew Ryan (voice)
Sander Cohen / Early Splicers (voice)
Atlas / Frank Fontaine (voice)
Yi Suchong / Bartender (voice)
Yi Suchong (as Thomas Gorrebeeck)
Rapture Citizen (voice)
Sex Without Compromise Narrator (voice)
(voice) (as Jim Bonney)
(voice) (as T.C. Carson)
(voice) (as Roger R. Cross)
Vox Populi (voice)
Public Service Announcements / Plasmid Narrator (voice) (as Elizabeth Foldes Meiman)
(voice)
Marsha Lutz / Leda / Little Sisters (voice)
Ryan Security / Early Splicers (voice)
(voice) (as Trisha Lynn Miller)
Ray Lardner / The Artist's Struggle Shopkeeper / Watched Clock Waiter / Rapture Citizen / Early Splicers (voice)
Big Daddy (voice)
Circus of Values Clown / Dollar Bill / Watched Clock Waiter (voice) (uncredited)
El Ammo Bandito (voice) (uncredited)