Miles Morales (voice)
Peter B. Parker (voice)
Gwen Stacy (voice)
Uncle Aaron (voice)
Jefferson Davis (voice)
Aunt May (voice)
Rio Morales (voice)
Mary Jane (voice)
Spider-Ham (voice)
Peni Parker (voice)
Spider-Man Noir (voice)
Doc Ock (voice)
Wilson Fisk (voice)
Peter Parker (voice)
Miss Calleros (voice)
Brooklyn Visions Security Guard (voice) (as Edwin H. Bravo)
Interesting Person #1 (voice)
Interesting Person #2 (voice)
Stan (voice)
Green Goblin / Last Dude (voice)
Scorpion (voice)
Tombstone (voice)
Scientist In Cafeteria (voice)
Vanessa Fisk (voice)
Brooklyn Friend (voice) (as Jessica Mikayla Adams)
Brooklyn Friend (voice) (as Gredel Berrios-Calladine)
Brooklyn Friend (voice)
Brooklyn Friend (voice) (as Kelby Alex Joseph)
Brooklyn Visions Student (voice)
Brooklyn Visions Teacher (voice)
Brooklyn Bystander (voice)
Additional Voices (voice)
Additional Voices (voice) (as Alycia L. Cooper)
Additional Voices (voice) (as Terrence Hardy Jr.)
Additional Voices (voice) (as Miguel B. Jiron)
Additional Voices (voice) (as Jason Linere White)