- He is a keen photographer and is always taking photos on the set of Supernatural (2005).
- Sings and plays guitar.
- Was a child model at age four, then quit, and started again at age ten.
- Planned to study sports medicine at Texas Tech University and become a physical therapist, before he decided to move to L.A. to give acting a try.
- Had his first daughter with wife Danneel Harris Ackles, named Justice Jay "JJ" Ackles, born on May 30, 2013.
- Was offered a role as Eliza Dushku's love interest on Tru Calling (2003) but chose to take a role on Smallville (2001) instead. Eric Christian Olsen was eventually given the role and the character's name was changed to Jensen.
- He has English, German, and smaller amounts of Scottish and Irish, ancestry. Many of his ethnic German ancestors lived in Russia.
- Ranked #39 on Empire Online list of the "100 Sexiest Movie Stars" in 2013.
- His career has an ongoing theme of dealing with demonic forces. His character's mother "Marlena" on Days of Our Lives (1965) was possessed by the Devil. In Devour (2005) he was the son of "Satan". On his television series Supernatural (2005) his character battles the forces of hell.
- His parents named him Jensen because they considered other names, such as Justin too common.
- On December 2, 2016, his wife Danneel Ackles gave birth to twins Zeppelin Bram and Arrow Rhodes.
- In 2016 announced that he and wife Danneel Ackles are expecting twins.
- Played baseball and lacrosse in high school.
- Was runner up to Tom Welling for the role of Clark Kent on Smallville (2001). Was brought onto the show in Season 4 as Lana's boyfriend.
- Has one brother, Joshua, approximately three years older, and one sister, Mackenzie, approximately seven years younger.
- His first name is actually a surname and is the most common surname in Denmark.
- Has sung lead vocals on two songs on the albums "Sharing the Covers" and "Christmas With Friends".
- Graduated from L.V. Berkner High School, Richardson, TX, in 1996.
- Preparing to perform next month on stage with Lou Diamond Phillips in "A Few Good Men". (May 2007)
- 1993 graduate of Apollo Junior High School in Richardson, TX.
- Son of Alan Ackles and Donna Joan Shaffer.
- Has worked with his girlfriend, later wife, Danneel Ackles twice: in Ten Inch Hero (2007) and in Supernatural (2005).
- On the set of Cheaper by the Dozen (2003), Jared Padalecki met Tom Welling, who played Clark Kent on Smallville (2001). Before the start of Supernatural (2005), Jensen also played on "Smallville".
- Jensen and longtime friend and musician Steve Carlson formed the band, Radio Company, in 2018. They have went on to have great success with their albums, Volume One released in November 2019 and Volume Two in May 2021.
- Auditioned and was considered for Clint Barton/Hawkeye in Thor (2011), Steve Rogers/Captain America in Captain America: The First Avenger (2011), Peter Quill/Star-Lord in Guardians of the Galaxy (2014) and Wade Wilson/Deadpool in Deadpool (2016).
- Jared Padalecki mentioned in an interview with TV Guide that the initial plan for Walker (2021) was that he would only executive produce while Jensen would play the lead. Jared ending up playing Walker as well.
- Voiced Bruce Wayne/Batman in the Tomorrowverse franchise but was considered for the character in Batman v Superman: Dawn of Justice (2016), Batwoman (2019) and Gotham Knights (2023).
- 1990 graduate of Dartmouth Elementary School in Richardson, TX.
- Before being cast as Dean Winchester on Supernatural (2005), he originally auditioned for Sam Winchester, and was the showrunners' favorite for the role before Jared Padalecki auditioned.
- Turned down to appear as Leon S. Kennedy three times in Resident Evil: Extinction (2007), Resident Evil: Afterlife (2010) and Resident Evil: Retribution (2012) due of working on Supernatural (2005).
- Graduated from Berkner High School with Stephanie Ware and Curtis Thomas in 1996.
- He had Lasik eye surgery to correct his myopia in 2009.
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