The man with the oboe-like instrument is playing the first phrase of "The Music Goes 'Round and 'Round."
Matri-Phony is the first The Three Stooges film to employ the accordion-based, driving version of 'Three Blind Mice' over the opening credits. This faster theme would be used until the end of 1944.
This was the first of two shorts directed by frequent The Three Stooges writer Harry Edwards (Three Little Twirps (1943) was the other one), but the experience was not very pleasant. Edwards was deemed an incompetent director by the cast and crew. Edwards had a explosive attitude towards the cast saying they couldn't act, etc. It was also the only short where stuff was filmed over a period of weeks, due to this. Del Lord was brought in to shoot more footage for the film, as a result.
Until the final shooting script, Larrycus was intended for the drag role.
Curly is in drag as a soon to be queen to the King-Vernon Dent. Moe tells Curly to go ahead a get sexy. This is the 1st time that anyone can ever recall the word sexy being spoken of in the cinema of the day.