Victor Maddern got a headache from the extensive makeup he had to wear as the deformed hunchback Carl.

According to a contemporary newspaper advertisement, the Moonlite Drive-In in Smithtown, Pennsylvania, on Wednesday, 3 August 1960 ran a dawn-to-dusk triple feature with Blood of the Vampire (1958) as the first movie, The Revenge of Frankenstein (1958) second, and The Beast of Hollow Mountain (1956) third. As a promotion, attendees whose tickets ended in 13 or 31 were treated to 'Dracula's Buffet Luncheon,' which consisted of 'Dracula's Cocktail, Deviled Zombie Skulls, Crispy Skull Chips, Devil Fruit, Voodoo Spirits' and a 'Werewolf Tall Sandwich'.

Magnetic Video's VHS release had excellent full color, released in 1978. In the 1980s it was thought that no good color print of the film had survived, but this release was being overlooked. Kodak Eastman color film was introduced in the 1950s and it was not designed to last more than a few short years, for what they called the normal circulation life of distributed films. As years went by everyone got angry at Kodak when the prints turned reddish. Kodak then in the 1980s improved it to last over 100 years. The original negative films did not fade, but they had to be transferred to positive prints for further releases.

Shooting began October 21 1957, shortly before Hammer started filming Horror of Dracula (1958). Both features were scripted by Jimmy Sangster.

Magnetic Video's edition is the first horror film released in the VHS format.

The film was released on a double bill with Universal's Monster on the Campus (1958) in October 1958.

The model village scene for Carlstadt, at about 10 minutes into the film, uses the same model set (with the Globe Theatre) as used for the film Henry V (1944).

Posters for Blood of the Vampire (1958) indicate that it was considered an adults-only film in France and the UK at the time of its release. French posters note that viewing by people under age 16 was prohibited and UK posters show that the film carried an X Certificate from the British Board of Film Censors, which prohibited the exhibition of the movie to those under 16. The X Certificate is indicative of the activities of Eros Film Distributors, which had by then deliberately 'embarked on a new X-certificate path'. Tempean Productions 'embraced' not only films designed to get an X cert, but also 'Eros's policy of offering co-feature programmes which could be marketed not only in Britain, but also on the American drive-in circuits'.

The film was inspired by the success of Hammer's The Curse of Frankenstein (1957) and Horror of Dracula (1958). The producers hired Jimmy Sangster, writer of both those movies, to do the screenplay. Shooting took four weeks.

Blood of the Vampire (1958) has been available for home viewing for decades in the USA. It was first released on VHS and Betamax by Magnetic Video in 1978. Gorgon Films released the movie, also on VHS, in 1993. A DVD release followed in 2006 from Dark Sky Films. Theatrical trailers from the film were used in the VHS tape Nightmare Theater's Late Night Chill-o-rama Horror Show Vol.1 in 1996. Coming by the film in the UK seems to have been more difficult. Hardy wrote in 1986 that 'all prints of it appear to have been destroyed'. The first mention of it being available for home viewing in the UK is the release of it on DVD by Simply Media in 2007.

Many fans thought the film was a Hammer Film when it came out, due to its similar look and Sangster's writer's credit.

This was the first attempt by the team of Robert S. Baker and Monty Berman to imitate, and hopefully duplicate the success of, the highly popular horror productions of Hammer Films. They hired Hammer team member and frequent screenwriter Jimmy Sangster to write the script.