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At the beginning of the movie, Spence is wearing a Leather jacket, which he gives to Alice. As the movie progresses, the jacket does not look big on Alice and even looks like a ladies size.
It seems that no one's handwriting matches that on the note, not even the handwriting of the note's original author (as seen in flashback).
The dress on the bed is not the dress Alice wears.
(at around 40 mins) When Matt kneels down to examine the blood of the undead Hive worker, he claims that blood only coagulates when you're dead. In truth, coagulation is simply the process of blood clotting around a damaged vessel wall, which indeed happens in living beings. On the contrary, in death, blood doesn't coagulate, but separates, causing livor mortis.
(at around 58 mins) The Red Queen tells the survivors that the human body remains active even during death, producing new cells such as hair and nails. This is a common misconception, as the skin of a dead body gradually pulls away from hair and nails as the body dehydrates, giving the appearance of the hair and nails growing. No new cells can be produced by a dead organism. Furthermore, neither hair nor nails are made up of cells; they are made primarily of a protein called keratin from dead epidermal cells.
(at around 2 mins) At the beginning of the movie, where the vial containing the virus is dropped, the fumes escape into the building through an air duct. This is not plausible, as any clean room that requires people to work in safety suits would be sealed, and therefore not have air vents.
(at around 8 mins) At the beginning of the movie, you can see the floor outside the elevator bending under the woman's weight as she tries to escape the elevator.
(at around 5 mins) When the lab at the beginning of the movie is filling with water, the camera scans the female scientist and her name shows up as Dr. Anna Bolt. Anna Bolt is the actress' name while her character is Dr. Green.
(at around 49 mins) The zombie in the kennel, after being kicked back (as well as before that) can be seen breathing.
When the tactical team first enters the house above the Hive, they are all wearing gas masks, however, none of them has filters attached and would, therefore, be useless.
(at around 24 mins) As the swat team makes its way through the flooded labs they stop and in a group shot Spence can be seen mouthing the words "What happened here?" but there is no sound. In the next shot he says it again this time with audio.
(at around 20 mins) When Kaplan says, "accessing schematics of the hive", you can see for a split second his mouth not moving to the dialogue.
Crew or equipment visible
(at around 50 mins) When Alice runs along the kennel wall to leap up and kick the last dog, you can clearly see a black wiring harness used for the stunt, under her jacket and dress.
When they are first entering the hive and the captain is speaking to Alice, a sound microphone is clearly visible at the top of the screen.
When Alice tries to retrieve the anti-virus box on the train platform, there is an observation camera shot from above with filming equipment (probably a microphone) visible at the bottom of the screen.