A viewer notified media watchdog Reverend Donald Wildmon that, in one episode of this series, it looks like Mighty Mouse reaches into a pocket and snorts cocaine from his hand to regain his strength. Wildmon was disbelieving at first, but after investigating the episode and learning of producer Ralph Bakshi's background (e.g., directing the adult cartoon Fritz The Cat), alerted the media that this may have been intentional. Given the numerous other instances of risque humor and adult jokes in this series, a drug reference wouldn't be out of the question. Bakshi denies it to this day, maintaining that Mighty Mouse was merely smelling some crushed flowers and that the white jet leading from his hand to his nose was merely a cartoon "smell line" moving super-fast from the mighty inhale. The episode was immediately pulled from the rotation so that no one would see it.

The episode "Mighty's Benefit Plan" features a singing group called Elwy and the Tree Weasles, an obvious lampoon of Alvin and the Chipmunks. Their creator, Ross Bagdasarian, was unflatteringly represented by the character Sandy Bottomfeeder. Ralph Bakshi used to work with Bagdasarian and did not get along with him. The constant appearance of cheerios coming out of Sandy's mouth is a reference to Honey Nut Cheerios and their aggressive promotion of The Chipmunk Adventure movie of the 80's.

Patrick Pinney, Charlie Adler, Rodger Bumpass, Bill Farmer, Mona Marshall, Jim Ward, Candy Candido, and Frank Welker also all appeared in Walt Disney Animation Studios films directed by John Musker and Ron Clements, Candido only appeared in their directorial debut The Great Mouse Detective (1986), Bumpass, Farmer, and Ward appeared in both Hercules (1997) and Treasure Planet (2002), Marshall appeared in both Treasure Planet (2002) and The Princess and the Frog (2009), Pinney and Adler appeared in The Little Mermaid (1989), Aladdin (1992), Hercules (1997), and Treasure Planet (2002), and Welker appeared in every single one of these with the exceptions of Treasure Planet (2002) and Moana (2016).