When Anne's family are being loaded into the truck after their capture, and again boarding the train their breath is seen condensing as if it's a cold winter's day, yet this event occurred in early August. Similarly, when Otto Frank arrives back in Amsterdam 'one month after the liberation' (according to the caption) his breath is again seen condensing as he leaves the train and again by the canal, and the trees are clearly bare, indicating winter, yet 'one month after the liberation' would be early/mid-June.
Anne is given the tattoo number A-25063. It is only seen when it is given to her and it not seen afterward, not even when her hair is being cut off.
When everyone begins disembarking from the cattle cars upon arriving at Auschwitz, Edith Frank can be seen leaving the car on two separate occasions.
In the movie, the address given to the Germans of the hiding place over the telephone by the supposed informer is incorrect. The informer says the address is 263 Lindtstradt, but was actually 263 Prinsengracht. It still exists today as the Anne Frank House museum in Amsterdam. The real betrayer of the hiding place has never been revealed or proven beyond only circumstantial evidence. The informer depicted in the movie is based on the belief of Melissa Muller, who wrote the book (Anne Frank: A Biography) that the movie is largely based on. In her book "The Hidden Life of Otto Frank" by Carol Ann Lee, which was published in 2002 and revised in 2003, an entirely different theory as to the identity of the informer is presented. Officially, the identity of the actual informer that betrayed those in the hiding place has never been conclusively determined and most likely never will be, as most of those that would be able to shed more light on the subject have since died.
The film implies a long-standing crush on Anne by Peter van Pels, even before the two went into hiding together. There is no evidence of this, and Anne hardly mentions Peter in her diary at all until January 1944 - a year and a half after going into hiding - when she expresses the overwhelming desire to talk to someone. Prior to this, Anne gives the reader the impression that she and Peter usually ignored each other.
During one of the air-raid scenes, Anne expresses hatred for all Germans. Otto reminds Anne that she is German, and she expresses surprise over this statement, asking Margot to confirm that they were Dutch. Actually, Anne knew full well that she was German, and expressed several times in her diary her sorrow over her "fatherland" being hijacked by the Nazis.
Otto Frank's cousin Milly asked for Margot and Anne to be sent to London, not his sister, Leni, who lived in Switzerland.
The movie depicts Edith Frank telling Margot that her call-up notice was for Otto, who thus relays this message to Anne. In reality, Margot knew that the call-up notice was for herself, and told Anne that it was for their father to break the news to her gently, but she later told Anne the truth.
The German guards in the camp watchtower are equipped with post-war Russian DHSK machine-guns.