The Batman / Bruce Wayne (65 episodes, 2004-2008)
Alfred Pennyworth / Alfred (50 episodes, 2004-2008)
Dick Grayson / Robin (24 episodes, 2006-2008)
Batgirl / Barbara Gordon / Moira (22 episodes, 2005-2008)
The Joker / Joker / Announcer / Instructor / Judge Wigzell (20 episodes, 2004-2008)
Detective Ellen Yin (17 episodes, 2004-2005)
Commissioner James Gordon / Commissioner Gordon (15 episodes, 2005-2007)
The Penguin / Penguin (14 episodes, 2004-2008)
Detective Ethan Bennett / Clayface / Ethan Bennett (11 episodes, 2004-2006)
Mayor Grange (7 episodes, 2004-2006)
Mr. Freeze / Lex Luthor / Fries (6 episodes, 2004-2007)
Chief Angel Rojas / Chief Rojas (6 episodes, 2005)
Catwoman / Selina (5 episodes, 2004-2007)
Professor Hugo Strange / Toyman (5 episodes, 2006-2008)
Firefly / Boy #1 / Boy #2 (5 episodes, 2004-2007)
Killer Croc / Bane / Rumor (4 episodes, 2005-2008)
Superman / Clark Kent (4 episodes, 2007-2008)
Martian Manhunter (4 episodes, 2007-2008)
Clayface / Metallo / Riddleman #1 / Tech 2 (4 episodes, 2007-2008)
Amanda / Amanda Raymont / Amber / Mary Grayson / Ms. Vimtrup / Vulture (4 episodes, 2004-2008)
Ragdoll / D.A.V.E. / Killer Moth / News Anchor (3 episodes, 2005-2006)
The Riddler / Riddler (3 episodes, 2005-2007)
Green Arrow (3 episodes, 2007-2008)
Dr. Kirk Langstrom / Langstrom (3 episodes, 2004-2008)
Professor Hugo Strange (3 episodes, 2005)
Poison Ivy / Pam Isley (3 episodes, 2005)
Scarface / Arnold Wesker / Mr. Snoots / Wesker (3 episodes, 2004-2008)
Black Mask (3 episodes, 2006-2008)
Captain Slash / Number 1 / Sands / Shadow Thief (3 episodes, 2006-2008)
Lucius Fox (3 episodes, 2007)
Green Lantern (3 episodes, 2007-2008)
Flash (3 episodes, 2007-2008)
Cash Tankinson (2 episodes, 2005-2006)
Harley Quinn (2 episodes, 2007)
Mel / Yelena (2 episodes, 2004-2005)
Freddy / Temblor / Vic (2 episodes, 2005)
Mirror Master (2 episodes, 2007-2008)
Lois Lane (2 episodes, 2007)
Mugsy / Rhino (2 episodes, 2004-2005)
Nightwing (2 episodes, 2007)
Cosmo Krank / Marty Slack / Toymaker (2 episodes, 2006-2007)
Crime Boss #2 / Guard / Reporter (2 episodes, 2004-2006)
Instructor / Ross / Speedway Announcer (2 episodes, 2004-2005)
Bomb Tech / Crime Boss #1 / Crime Boss #3 (2 episodes, 2004-2005)
Hawkman (2 episodes, 2008)
Andy Mallery / Harris / Scorn (2 episodes, 2007-2008)
Mercy (2 episodes, 2007)
Cluemaster (1 episode, 2004)
Donnie / Prank (1 episode, 2006)
Bert (1 episode, 2004)
Solomon Grundy (1 episode, 2005)
Everywhere Man / John Marlowe (1 episode, 2006)
Julie (1 episode, 2007)
David / Fox (1 episode, 2008)
Bane (1 episode, 2004)
Becky (1 episode, 2004)
Mrs. Brown (1 episode, 2004)
Katsu (1 episode, 2004)
Spellbinder (1 episode, 2005)
Gearhead (1 episode, 2005)
Kilgore Steed (1 episode, 2005)
Georgia / Virgina (1 episode, 2005)
Clea (1 episode, 2006)
John Grayson (1 episode, 2006)
Ewan (1 episode, 2006)
Francis Grey (1 episode, 2007)
Gorman (1 episode, 2007)
Sinestro (1 episode, 2007)
Count Vertigo (1 episode, 2007)
Justin / Shark (1 episode, 2008)
Sprang (1 episode, 2004)
Movie Star / Reporter / Wife (1 episode, 2004)
Rupert Thorne (1 episode, 2004)
Bagley (1 episode, 2005)
Brent (1 episode, 2005)
Duncan (1 episode, 2005)
Maximillion Zeus (1 episode, 2006)
Basil Karlo / Clayface II (1 episode, 2006)
Smoke (1 episode, 2007)
Blaise (1 episode, 2007)
Doctor / Socialite (1 episode, 2007)
Campus Guard (1 episode, 2007)
Mr. Herbert (1 episode, 2007)
Curator (1 episode, 2008)
Chief Rojas (1 episode, 2004)
Zucco (1 episode, 2006)
Lady / Reporter (1 episode, 2006)
Detective (1 episode, 2006)
Oracle (1 episode, 2007)
Worker #2 (1 episode, 2007)
Boy (1 episode, 2007)
Anchor / Doctor (1 episode, 2007)
Harriet (1 episode, 2007)
Female Caller (1 episode, 2007)
Kid (1 episode, 2008)
Thug #1 (1 episode, 2004)
Bruiser (1 episode, 2006)
Director / Waitress (1 episode, 2006)
Paul (1 episode, 2007)
Superior (1 episode, 2007)
Chuck (1 episode, 2007)
Jake (1 episode, 2008)
William Mallery / Wrath (1 episode, 2008)
Warden (1 episode, 2008)
Pilot (1 episode, 2007)
Number 1 (1 episode, 2008)
Jimmy Olsen (1 episode, 2007)
Sally Galt (1 episode, 2008)