Bruce Wayne / The Batman (65 episodes, 2004-2008)
Alfred Pennyworth (48 episodes, 2004-2008)
Dick Grayson / Robin (24 episodes, 2006-2008)
Batgirl / Barbara Gordon / Additional voices / Moira (21 episodes, 2005-2008)
The Joker / Announcer / Instructor / Judge Wigzell / The Joker 2.0 (20 episodes, 2004-2008)
Detective Ellen Yin (16 episodes, 2004-2005)
The Penguin / Oswald Cobblepot (14 episodes, 2004-2008)
Commissioner James Gordon (13 episodes, 2005-2007)
Detective Ethan Bennett / Clayface / Ethan Bennett (11 episodes, 2004-2006)
Mayor Grange (7 episodes, 2004-2006)
Chief Angel Rojas (6 episodes, 2005)
Mr. Freeze / Lex Luthor / Bane / Victor Fries (6 episodes, 2004-2007)
Firefly / Boy #1 / Boy #2 / Garfield Lynns / Phosphorus / Sports Commentator / Thug #2 (5 episodes, 2004-2007)
Catwoman / Selina Kyle (5 episodes, 2004-2007)
Ragdoll / D.A.V.E. / Jim / Killer Moth / News Anchor / Reporter (3 episodes, 2004-2006)
Professor Hugo Strange / Toyman (5 episodes, 2006-2008)
Clayface / Metallo / Hamilton Hill / Riddleman #1 / Tech 2 (4 episodes, 2007-2008)
Superman / Clark Kent (4 episodes, 2007-2008)
Amanda / Amanda Raymont / Amber / Mary Grayson / Ms. Vintrup / Vulture (4 episodes, 2004-2008)
Killer Croc / Bane / Rumor (4 episodes, 2005-2008)
Martian Manhunter (4 episodes, 2007-2008)
The Riddler (3 episodes, 2005-2007)
Dr. Kirk Langstrom (3 episodes, 2004-2008)
Scarface / Arnold Wesker (3 episodes, 2004-2008)
Professor Hugo Strange (3 episodes, 2005)
Captain Slash / Number 1 / Sands / Shadow Thief (3 episodes, 2006-2008)
Black Mask (3 episodes, 2006-2008)
Green Arrow / Oliver Queen (3 episodes, 2007-2008)
Poison Ivy / Pamela Isley (3 episodes, 2005)
Lucius Fox (3 episodes, 2007)
Green Lantern / Hal Jordan (3 episodes, 2007-2008)
The Flash (3 episodes, 2007-2008)
Mel Bramwell / Yelena Klimanov / Young Artie (2 episodes, 2004-2005)
Freddy / Temblor / Vic (2 episodes, 2005)
Lois Lane (2 episodes, 2007)
Robinson Sprang (1 episode, 2004)
Bomb Tech / Crime Boss #1 and Crime Boss #3 (2 episodes, 2004-2005)
Mercy Graves (2 episodes, 2007)
Mugsy / Rhino (2 episodes, 2004-2005)
Harley Quinn / Dr. Harleen Quinzel / Harleen Quinzel (2 episodes, 2007)
Hawkman (2 episodes, 2008)
Cash Tankinson (2 episodes, 2005-2006)
Nightwing (2 episodes, 2007)
Crime Boss #2 / Guard / Reporter (2 episodes, 2004-2006)
Ross Darren / Speedway Announcer (2 episodes, 2004-2005)
Cosmo Krank / Marty Slack / Toymaker (2 episodes, 2006-2007)
Andy Mallery / Harris / Scorn (2 episodes, 2007-2008)
Factory Scientist / General (1 episode, 2006)
Mirror Master (2 episodes, 2007-2008)
Arthur 'Artie' Brown / The Cluemaster (1 episode, 2004)
Herbert Ziegler (1 episode, 2004)
Solomon Grundy (1 episode, 2005)
Bane (1 episode, 2004)
Becky (1 episode, 2004)
Mrs. Brown (1 episode, 2004)
Hideo Katsu (1 episode, 2004)
Georgia / Virgina (1 episode, 2005)
Spellbinder (1 episode, 2005)
Killgore Steed (1 episode, 2005)
Count Vertigo (1 episode, 2007)
Rupert Thorne (1 episode, 2004)
Moviestar / Reporter / Wife (1 episode, 2004)
Bagley (1 episode, 2005)
Brent (1 episode, 2005)
Duncan (1 episode, 2005)
Basil Karlo / Clayface II (1 episode, 2006)
Blaise (1 episode, 2007)
Doctor / Socialite (1 episode, 2007)
Curator (1 episode, 2008)
Chief Angel Rojas (1 episode, 2004)
Lady / Reporter (1 episode, 2006)
Harriet (1 episode, 2007)
Anchor / Doctor (1 episode, 2007)
Thug #1 (1 episode, 2004)
Director / Waitress (1 episode, 2006)
Chuck (1 episode, 2007)
Warden (1 episode, 2008)
William Mallery / Wrath (1 episode, 2008)
Number 1 (1 episode, 2008)
Jimmy Olsen (1 episode, 2007)
Sally Galt (1 episode, 2008)
Chlorogene Employee / Officer 1 (1 episode, 2005)
Gearhead (1 episode, 2005)
Mind Controlled Officer (1 episode, 2005)
Ewan (1 episode, 2006)
John Grayson (1 episode, 2006)
Thug with Utility Belt (1 episode, 2006)
Tony Zucco (1 episode, 2006)
Bruiser (1 episode, 2006)
Maximillion Zeus (1 episode, 2006)
Teacher (1 episode, 2006)
Clea (1 episode, 2006)
Donnie / Prank (1 episode, 2006)
Man at the Bank (1 episode, 2006)
Everywhere Man / John Marlowe (1 episode, 2006)
Man screaming 'It's Joker' (1 episode, 2006)
Detective (1 episode, 2006)
Smoke (1 episode, 2007)
Paul (1 episode, 2007)
Campus Guard (1 episode, 2007)
Mr. Herbert (1 episode, 2007)
Sinestro (1 episode, 2007)
Francis Grey (1 episode, 2007)
Superior (1 episode, 2007)
Gorman (1 episode, 2007)
Pilot (1 episode, 2007)
Oracle (1 episode, 2007)
Female Caller (1 episode, 2007)
Worker #2 (1 episode, 2007)
Boy (1 episode, 2007)
Julie (1 episode, 2007)
David / Fox (1 episode, 2008)
Justin / Shark (1 episode, 2008)
Kid (1 episode, 2008)
Jake (1 episode, 2008)
Old Man (uncredited) (unknown episodes)
Arkham Warden / Citizan (uncredited) (unknown episodes)
Cop (uncredited) (unknown episodes)
Connie (uncredited) (unknown episodes)