In Dante Alighieri's the Divine Comedy, Dante and Vergil happen across the Cerberus on the third level of hell. Dante encounters Cerberus at the end of Mission 3 in the game.

The opening cutscene for every mission contains a number which represents the mission number. For example, the pizza box from mission one has a '1' printed on it, the '2' on the billboard in mission two, etc.

Rebellion was the key to unlocking Dante's "Devil Trigger." Sparda enchanted the sword to react with Dante's own blood in order to bring out his power. (Prior to his battle with Vergil, the eyes of the skull on Rebellion were closed and the bottom of the handle was just a pointed edge. But after the battle, the eyes blowed and the bottom of the handle was opened.)

Though the blade Force Edge and both of Sparda's amulets are featured in the game, the 3 are never joined to create the sword "Sparda." (Which would bestow the wielder with Sparda's latent powers.)

As trailers for the game reveal, the pool table in Dante's office was changed from a green to a red one at a very late moment in production

This game's description of Sparda's final defiant act against the underworld differs from the take told in the first game. This game tells that Sparta betrayed demonkind and sealed the portal to the underworld, with little mention of Emporer Mundus. The first game opens with a tale of how Sparta waged a one-man war against the entire underworld, defeating Mundus himself and then sealing demonkind (along with his own demonic power) in the underworld. The full story is never explained in a linear fashion and each new game adds some aspect of Sparda's story that was not previously mentioned.

While most cutscenes are rendered real-time, nearly every cutscene which shows different locations (or has large-scale happenings) is pre-recorded. For example, the collapse of the Leviathan onto the Temen-Ni-Gru is pre-recorded, as well as the opening sequence of mission one, which features both Dante's office's interior and exterior, as well as an elaborate fighting scene.

A press error led many people to believe that Ryûhei Kitamura was directing the game's cutscenes. 'Yuji Shimomura', who served as action director on Kitamura's film Versus (2000), is the actual cut-scene director.