The mad scientist who never speaks, is named Fritz Huhnmörder. "Huhnmörder" is German for "chicken murderer".

Over sixty titles were considered for this show, including "Junk in the Trunk", "ADD TV", "Gold Dust Gasoline", "Toyz in the Hood", "Vegetable Fun Fest", and "S&M Presents", but all were rejected due to trademark or other legal reasons. Out of the many titles the show's creators submitted, only "Robot Chicken", the name of a local Chinese restaurant dish, was accepted. Some of the rejected names were later used as episode titles.

In the credits of every episode, Sarah Michelle Gellar and Mila Kunis have a special spoof credit, and in most seasons, there is a different person from the staff given a spoof credit right after them.

In the opening credit sequence, the chicken receives cybernetic parts exactly like those of Vic "Cyborg" Stone from the Teen Titans of DC Comics.

The closing theme music is a chicken-clucking version of The Gonk, a piece of music played in the mall in Dawn of the Dead (1978) that was also used for the closing credits of the film.

Les Claypool is the composer of the theme song. He also composed and sang the theme to South Park (1997), and is the leader of the band, Primus.

The show is based on a regular feature in ToyFare Magazine, of which Matthew Senreich was the Editor. The feature was titled "Twisted ToyFare Theater" and had various toys in bizarre situations. Then, Senreich interviewed Seth Green for his magazine, and Green approached Senreich about doing an animated series based on the magazine feature. The result was an Internet series titled "Sweet J Presents", which aired on Sony's website in 2001. Green and Senreich then pitched the show to the Cartoon Network.

Although some stop-motion animation figures are made of clay, the figures in this series are either a hybrid of plastic doll limbs and Styrofoam torsos, or toys that are made completely of plastic. Very few small figures, however, like the lemmings in "The Animal Kingdom Presents" sketch, are made of clay.

Animator Dillon Markey uses a modified Nintendo Power Glove (by Mattel) with Bluetooth to control his laptop and some figures while animating segments.

In "Junk in the Trunk" during the Superman blooper reel, the picture on the wall, seen above the desk, is also seen in the second "Seacrest, out" skit from "Plastic Buffet".

All of the product placements are heavily blurred out for comedic purposes. There are no actual brands in the blurred out areas, they are nothing but plastic pieces of Lego or other toys. Somehow, the brand names of products and stores are frequently mentioned.

The first season contained seventy uses of the "f" word, whereas South Park (1997)'s first season only consists of about twenty.