Numbuh 4 - Wally Beatles / Mr. Fibb / Toilenator / Joaquin / Tommy Gilligan / Delightful Children from Down the Lane / Additional Voices (voice)
Numbuh 1 - Nigel Uno / Numbuh 2 - Hoagie P. Gilligan Jr. / Numbuh 101 - Matthew / Delightful Children from Down the Lane (voice)
Numbuh 5 - Abigail 'Abby' Lincoln / Cree Lincoln / Delightful Children from Down the Lane (voice)
Numbuh 3 - Kuki Sanban (voice)
Sector Z Numbuh 0.1 - Bruce (voice)
Mr. Boss / Mr. Fizz / Benedict Wigglestein (voice) (as Jeff Glen Bennet)
Additional Voices (voice)
Crazy Old Cat Lady / Gramma Stuffum (voice) (as Grey DeLisle)
Numbuh 86 - Francine 'Fanny' Fulbright / Numbuh 363 - Harvey McKenzie / Computer (voice)
Stickybeard (voice)
Chad Dickson (voice)
Numbuh 83 - Sonya / Numbuh 84 - Lee (voice)
Knightbrace / Chester / Mr. Wink / Sector Z Numbuh 0.2 - David / Additional Voices (voice)
Father (voice)
Numbuh 60 - Patton Drilovsky (voice)
Numbuh 362 - Rachael T. McKenzie / Sector Z Numbuh 0.3 - Ashley (voice)
Count Spankulot / Janitor (voice)
Grandfather (voice)
Monty Uno (voice)
Numbuh Zero / Sector Z Numbuh 0.4 - Lenny (voice)