The televisions in the Buy More store will always display images or footage that will relate to what is occurring within the episode. For example, in season one, episode two, "Chuck Versus the Helicopter", the televisions display footage of helicopters.

During an interview with The A.V. Club, Vik Sahay said that he first auditioned for the role of Morgan, and when the auditions came down to just him and Joshua Gomez, and Gomez got it, the producers instead offered Sahay the then-smaller role of Lester. Lester since became a more prominent character than he was in the pilot.

Chris Pratt auditioned to play Chuck Bartowski.

In a 2013 National Public Radio interview, Zachary Levi said that he was almost in the Broadway musical production of "Young Frankenstein", but he had to give up the opportunity, because the pilot for this show got picked up as a series.

Throughout the series, Chuck almost always wears classic black Converse Chuck Taylors, often referred to as "Chucks."

At the close of the second season, declining ratings and stiff competition from the other networks had indicated that Chuck would be canceled. However, due to petitioning from its loyal fan base specifically aimed at one of its sponsors (Subway), the show was renewed for a third season, in an unprecedented partnership between NBC and the sandwich chain helping to cover the production costs. Chuck continued to face cancellation for its subsequent seasons, with its fifth season becoming its final season, beginning October 28, 2011, and ending on January 27, 2012, with a two hour finale.

In season two, episode eleven, "Chuck Versus Santa Claus", there are several references to Die Hard (1988). The LAPD officer in charge is Al Powell (Reginald VelJohnson), who also eats Twinkies. He responds to the F.B.I. agent who asks "Who's in charge here?", just like in the movie. Al Powell also calls his cousin, the store manager, Mike Powell, and uses a line from the film, "How ya holding up in there, partner?"

Christopher Lloyd played Chuck's therapist. Chuck constantly referred to him as Doc. This referred to Lloyd's role in the Back to the Future trilogy.

In season four, episode twelve, "Chuck Versus the Gobbler", the prisoner number on Chuck's shirt is 092980, which is Zachary Levi's birthday.

Although the Buy More sells the latest home electronics, the television in the break room is a 1985 Hitachi.

After appearing in the vast majority of episodes from seasons one through three, Bonita Friedericy (General Beckman) was finally made a regular cast member in season four.

Natalie Martinez was to join the cast after the pilot episode as Kayla Hart, a love interest for Chuck. Martinez appeared in the cast promotional pictures, but her character was written out before she could appear on the show.

In season three, episode ten, "Chuck Versus the Tic Tac", the box where an important item was kept is number 092407, which is the date the show premiered, September 24, 2007.

In season two, episode twelve, "Chuck Versus the Third Dimension", Tony Hale yells "No Touching!", a popular quote from Arrested Development (2003), where he plays the character "Buster Bluth".

Bruce Boxleitner played Dr. Woodcomb, Devon's (Ryan McPartlin's) father. He also played the title role in TRON (1982). Chuck has a poster of Tron in his room.

Chuck's alter ego "Charles Carmichael" may be a reference to musician Hoagy Carmichael. Ian Fleming often described his character James Bond as looking like Hoagy Carmichael.

In season four, episode ten, "Chuck Versus the Leftovers", Chuck's mom (Linda Hamilton) says "Come with me if you want to live!". This is a direct quote from Arnold Schwarzenegger in Terminator 2: Judgment Day (1991), in which Hamilton also starred.

Daniel Shaw is played by Brandon Routh, who starred in Superman Returns (2006). At one point, Chuck refers to Shaw's physique as "Superman-y".

Many references are made to Spies Like Us (1985). The bug used in several episodes is called a GLG-20, which is the designation given to undercover operatives in the movie. Emmett Millbarge is named after the two main characters in the movie, first name taken from Chevy Chase's Emmett Fitz-Hume, and last name taken from Dan Aykroyd's Austin Millbarge; this is also the order they are introduced in the film. In an episode, Chuck poses as a doctor, and references a scene from the movie, where doctors go through greeting each other by saying "Doctor, Doctor". Chevy Chase had a guest role on the show. In the movie, one of the actors playing the Tadzhik Highway Patrolman has the name Costa-Gavras, and the name of the Communist country featured in season three, episode three, "Chuck Versus the Angel de la Muerte" has the name Costa Gravas. Also, while Chuck and Sarah are watching television, Spies Like Us (1985) comes on, and Sarah (Yvonne Strahovski) comments "not again". Another reference is from one of the episodes featuring Chevy Chase; in which is featured an underground bunker disguised as a drive-in movie theater.

In season two, episode five, "Chuck Versus Tom Sawyer", during her interview with Emmett (Tony Hale), Anna (Julia Ling) states that she speaks binary, and says "00011010" which is her age, twenty-six.

Sarah Lancaster, who plays Zachary Levi's older sister, Ellie, is seven months older than him.

Jeff's (Scott Krinsky's) full name is Thomas Jefferson Barnes.

Ellie's full name is Eleanor Faye Bartowski Woodcomb, MD, PhD.

In season five, episode three, "Chuck Versus the Frosted Tips", Morgan (Joshua Gomez) dodges the tranquilizer darts in the same fashion Neo (Keanu Reeves)) does in The Matrix (1999). Carrie-Anne Moss, who plays Gertrude Verbanski, played Trinity in the Matrix trilogy.

In season four, episode seventeen, "Chuck Versus the First Bank of Evil", there are actually two references to Lost (2004). The first is when Vivian (Lauren Cohan) receives the bank card at Volkoff Industries, in which the card displays the famous numbers from Lost (2004) 4815162342. The second reference is at the National Bank of Macau. Guillermo Chan, the head of the bank, is played by François Chau, who also played Dr. Pierre Chang from the Dharma Initiative in Lost (2004).

Yvonne Strahovski (Sarah Walker) and Ryan McPartlin (Dr. Devon "Captain Awesome" Woodcomb) provided the voices of Peg Mooring and Clutch Powers in Lego: The Adventures of Clutch Powers (2010) between seasons of this show.

In season two, episode eleven, "Chuck Versus Santa Claus", the gunman goes by the name Ned Ryerson, which is the name of a character played by Stephen Tobolowsky in Groundhog Day (1993).

Actor Brandon Molale guest stars in one episode. He lobbied many years for the role of Captain Marvel/Shazam which eventually went to Zachary Levi in Shazam! (2019).

In seasons one through three, Big Mike (Mark Christopher Lawrence) has a marlin on the wall of his office that features in several episodes. In season four, Morgan Grimes (Joshua Gomez) has a "Billy the Big Mouth Bass" on the wall behind his desk.

In season three, two new characters were introduced. Both actor and actress starred in a Superman-related television series and movie: Brandon Routh played Superman in Superman Returns (2006), and Kristin Kreuk played Lana Lang in Smallville (2001). And, Summer Glau who voiced Kara Zor-El/ Supergirl in the animated movie Superman/Batman: Apocalypse. Also, Adam Baldwin voiced Clark Kent/Superman in Superman: Doomsday.

Chuck's full name is Charles Irving Bartowski.

Yvonne Strahovski and Adam Baldwin also voiced characters in the video game series Mass Effect, specifically in Mass Effect 2, with a couple of appearances in Mass Effect 3. Strahovski played the ever lovely genetically engineered to be perfect Miranda and Baldwin the gung-ho Quarian Marine Kal'Reegar. Interestingly enough, their roles seem to have a bit of a parallel to that of Agents Walker and Casey.

When Dolph Lundgren appears in the season four opener, "Chuck Versus the Anniversary", he makes several references to his character Ivan Drago from Rocky IV (1985). He does so when interrogating John Casey (Adam Baldwin), telling him, "I must break you", as well as threatening the team saying, "If they die, they die."

Dr. Stanley Wheelwright (Robert Englund) has connections with nightmares, which ties in with his role as Freddy Krueger, in the "A Nightmare on Elm Street" franchise.

In several episodes, Chuck makes or refers to chicken pepperoni, which is a dish Chevy Chase made in Seems Like Old Times (1980).

Sarah Walker is also used as a codename for Sarah Davies (Kristin Scott Thomas) in Mission: Impossible (1996).

The foyer of Ted Roark's (Chevy Chase's) building is the same used as Globo Gym in Dodgeball (2004).

Matt Bomer and Tim DeKay had recurring roles. They also starred in White Collar (2009) during the same time.

Morgan Grimes's middle name is Guillermo.

Christopher Lloyd plays Chuck's therapist, and goes to a clinic, referring to Lloyd's role in One Flew Over the Cuckoo's Nest (1975).

Sarah Walker's middle name is Lisa.

Josh Schwartz included the fictional Yakuza Prep poster from Adam Brody's room in The O.C. (2003) in Zachary Levi's room.

Both Zachary Levi (Chuck Bartowski) and Brandon Routh (Daniel Shaw) have played comic book characters in movies. Stan Lee, who appears in an episode, was a comic-book writer, editor, and publisher, who was executive vice president and publisher of Marvel Comics. Zac played Fandral in Thor: The Dark World (2013) and Thor: Ragnarok (2017), then he played Shazam in Shazam! (2019) and Shazam! Fury of the Gods (2023). Brandon played Clark Kent/Superman in Superman Returns (2006), Todd Ingram in Scott Pilgrim vs. the World (2010) and Scott Pilgrim vs. the World Water Crisis (2020), then played Ray Palmer/The Atom/Neron/Superman/Superman (Earth-96)/Clark Kent (Earth-96) in Arrow (2012), The Flash (2014), Supergirl (2015), DC's Legends of Tomorrow (2016) and Batwoman (2019).

In the season four opener, Chuck (Zachary Levi) and Morgan (Joshua Gomez) constantly use "Tangiers" as their code word to run. This is possibly a reference to The Bourne Ultimatum (2007), when Jason Bourne is in Tangiers, Morroco, and there is a lot of running.

Guest stars Linda Hamilton, Robert Patrick, Summer Glau, and Brian Thompson have appeared in the Terminator franchise. Linda Hamilton played Sarah Connor in The Terminator (1984) and Terminator 2: Judgment Day (1991). Robert Patrick played the T-1000 in Terminator 2: Judgment Day (1991). Summer Glau played Cameron Phillips in Terminator: The Sarah Connor Chronicles (2008), and Brian Thompson played a punk in The Terminator (1984). Cast member Mark Christopher Lawrence was also in Terminator 2: Judgement Day (1991), and had a scene with Linda Hamilton.

Harry Tang's (C.S. Lee) complete name is Harold Tiberius Tang, a reference to Captain James Tiberius Kirk.

Scott Bakula (Steven Jay Bartowski) and Brandon Routh (Daniel Shaw) share the same birthday.

Brandon Routh (Daniel Shaw) and Jordana Brewster (Dr. Jill Roberts) are distant cousins. They're both descended from William Brewster (c. 1566-1644), a noted Pilgrim, and wife Mary, who came over to Massachusetts, America, as Mayflower passengers.

Adam Baldwin and Christopher Lloyd appeared in InSight (2011).

Mark Christopher Lawrence and Christopher Lloyd appeared in Blood Lake: Attack of the Killer Lampreys (2014).

Zachary Levi (Chuck) played Fandral in Thor: The Dark World (2013).

Ryan McPartlin (Dr. Devon "Captain Awesome" Woodcomb) and Summer Glau (Greta) appeared in Sequestered (2014).

Tony Todd (C.I.A. Director Graham) and Robert Englund (Dr. Stanley Wheelright) appeared in Hatchet (2006).

Both Ryan McPartlin (Devon 'Captain Awesome' Woodcomb) and Brandon Routh (Daniel Shaw) have appeared in Sugarland music videos. Ryan appeared in 'Stuck Like Glue', that was released in 2010 and Brandon appeared in 'Babe' featuring Taylor Swift, that was released in 2018. Taylor famously dated Tom Hiddleston back in 2016, who has worked with Zachary Levi (Chuck Bartowski) in Thor: The Dark World (2013) and Thor: Ragnarok (2017). They played Loki and Fandral respectively.

Chuck's father (played by Quantum Leap (1989)'s Scott Bakula) says his trademark line from the time travelling show, when meeting Chuck and Sarah for the first time. "Oh, boy!"

In season one, episode two, "Chuck Versus the Helicopter", Chuck is listing a series of things he knows from the intersect. One of them is a reference to Lost (2004), when he says "Oceanic Flight 815 was shot down by a surface to air...."

In the series finale, the movie on television, that shows cowboys riding into the sunset, is Silverado (1985).

In season three, episode eighteen, "Chuck Versus the Subway", Alex (Casey's daughter) runs away from Casey (Adam Baldwin) after he tries to save her and he yells, "I am your father!". Alex (Mekenna Melvin) responds with "That's impossible!", which is a reference to Star Wars: Episode V - The Empire Strikes Back (1980).

In the final episode of season two, Chuck (Zachary Levi) uploads the Intersect 2.0 in his brain. He then proceeds to take out members of The Ring by flashing Kung Fu with his new Intersect. After beating them up he says "Guys, I know Kung Fu" a direct quote from The Matrix (1999).

In season four, episode nine, "Chuck Versus Phase Three", Richard Chamberlain plays "The Belgian", who wanted to take away Chuck's memories, emotions, and personality, making him one hundred percent spy. Richard Chamberlain starred in The Bourne Identity (1988) as Jason Bourne, a spy who lost his entire memory and personality, and became one hundred percent spy.

In season three, episode seven, "Chuck Versus the Mask", Chuck goes to a building to save Shaw (Brandon Routh). The building that was used is the same building used in Mighty Morphin Power Rangers (1993), as the command center.

In the beginning of season four, episode five, "Chuck Versus the Couch Lock", three men show up to John Casey's funeral and proceed to provoke his body to make sure he's dead. This is a reference to Charade (1963), in which the same three methods are used to wake up another dead man at his funeral.

In season four, episode twelve, "Chuck Versus the Gobbler", Sarah (Yvonne Strahovski) says, "I love a good suicide mission", which is possibly a reference to her character Miranda Lawson in Mass Effect 2 (2010).

Chevy Chase is mentioned by Chuck when watching a film. He also played the role of Ted Roark.