Optimus Prime / Lugnut / Grimlock / Cliffjumper / Highbrow / Security Bot / Sparkplug Witwicky / News Bot / Warpath (42 episodes, 2007-2009)
Prowl / Captain Fanzone / Ultra Magnus / Mixmaster / Angry Archer / Soundwave / Condiment Bot / Grandus / Sparkplug Witwicky (39 episodes, 2007-2009)
Bumblebee / Blitzwing / Porter C. Powell / News Anchor / Blackout / Computer Voice / Worker (39 episodes, 2007-2009)
Isaac Sumdac / Starscream / Scrapper / Jetfire / Ramjet / Tutor Bot / Sunstorm / Skywarp / Thundercracker / Wasp / Commercial Announcer / Cop / Rattletrap / Starscream Clones / Waspinator (36 episodes, 2007-2009)
Bulkhead / Driver / Hot Shot / Master Disaster (36 episodes, 2007-2009)
Ratchet / Megatron / Shockwave / Longarm Prime / Ironhide / Cyrus 'Colossus' Rhodes / Longarm / Man / Spike Witwicky (35 episodes, 2007-2009)
Sari Sumdac / Slipstream / Mayor's Aide / Strika / Teletran-1 / Carly Witwicky / Daniel Witwicky / Gary / Receptionist Bot / Red Alert / Slo-Mo (34 episodes, 2007-2009)
Sentinel Prime / News Bot / Tracks (13 episodes, 2007-2009)
Jazz / Jetstorm / Omega Supreme / Alpha Trion / Oil Slick (12 episodes, 2008-2009)
Blackarachnia / Elita-1 (6 episodes, 2007-2009)
Headmaster / Henry Masterson (4 episodes, 2008-2009)
Arcee / Flareup (4 episodes, 2008-2009)
Blurr (4 episodes, 2008-2009)
Lockdown (3 episodes, 2008-2009)
Meltdown / Prometheus Black (3 episodes, 2008)
Professor Princess / Police Dispatcher / Trisha (2 episodes, 2008)
Swindle (2 episodes, 2008-2009)
Wreck-Gar / Sumdac Employee (2 episodes, 2008-2009)
Nanosec / Nino Sexton (2 episodes, 2008)
Omega Supreme (1 episode, 2008)
Rodimus Prime (1 episode, 2009)
Yoketron (1 episode, 2009)
Dirt Boss (1 episode, 2009)