The pet cemetery features the grave of Zero from The Nightmare Before Christmas (1993).

The turtle "Shelley" is named for Mary Shelley, who wrote "Frankenstein: or, The Modern Prometheus," the 1818 novel on which this film is loosely based.

Victor's friend and next-door neighbor is Elsa Van Helsing, a reference to Abraham Van Helsing, Bram Stoker's Dracula character, and Elsa Lanchester, the original The Bride of Frankenstein (1935).

This is Tim Burton's first film since Big Fish (2003) not to feature Johnny Depp, his first since Sleepy Hollow (1999) not to feature Helena Bonham Carter and his first since Mars Attacks! (1996) to feature neither of them.

The mayor of the town is Mr. Burgermeister, which means "mayor" in German. His name/appearance is also a homage to Burgermeister Meisterburger, the antagonist of the stop-motion film Santa Claus Is Comin' to Town (1970).

Although Christopher Lee had appeared in two previous films by Tim Burton. He does appear in archive footage of him in Horror of Dracula (1958) is watched by Victor's parents on television.

The hair on the head of Elsa Van Helsing's dog Persephone is copied from the hair of Elsa Lanchester's character in The Bride of Frankenstein (1935) right down to the white streaks.

The film's release spawned a fan theory that this film, and Burton's other two stop motion animated films, The Nightmare Before Christmas (1993) and Corpse Bride (2005), all are taking place in a shared continuity, and tell one big story spanning several centuries, and the possibility that Victor Frankenstein and Sparky are descendants of Victor Van Dort and Scraps from Corpse Bride (2005).

Winona Ryder and Tim Burton's third collaboration, with gap of 22 years since their second one, Edward Scissorhands (1990).

Mr. Rzykruski is directly modeled on Martin Landau, whose character is voiced by Landau. Not only was Martin right for the part, but he has appeared in a number of previous Burton films, including Ed Wood (1994), for which he won an Oscar for best supporting actor.

The demonstration that Mr. Rzykruski shows in class of the frog's legs twitching when given electricity is based on actual experiments in 1771 by Italian physicist Luigi Galvani, who was the first to discover that the legs of dead frogs and other dead creatures twitched and moved when sparked by electricity. This led to the study of bio-electricity and further study of the nervous system and its functions. The study of "galvanic" effects in biology is named after Galvani, who is seen as the discoverer of bio-electricity. Several of Tim Burton's movies have played with this theme, most notably Frankenweenie and Edward Scissorhands (1990).

This film reunites Tim Burton with Winona Ryder and Catherine O'Hara who previously worked with Burton on Beetlejuice (1988).

Nassor is strongly similar in appearance and speech to the original monster from Frankenstein (1931) while his hamster is a Mummy. Both Frankenstein's monster and The Mummy (1932) were played by the actor Boris Karloff.

Winona Ryder did her own singing. She agreed to do it when Tim Burton said the singing had to sound imperfect.

Tim Burton stated in an interview for the CBBC show Blue Peter (1958) that if the studio had said they would not permit him to do the film in black and white, he would leave the project as he felt it reflected the classic horror movie style of the the film.

While Tim Burton based the town of New Holland on a similar town in which he grew up outside Burbank, California, Disney officially chose New Holland, Pennsylvania, as the town where the film takes place. After the principal of New Holland Elementary School (also the name of the school in the film) contacted Burton and the film's producers about the fictional town's similarities to New Holland, PA, Disney representatives brought movie segments and promotional materials to the school. Disney representatives also brought a parade float to New Holland's fall festival. New Holland, PA, is also known for its Pennsylvania Dutch background and holds a fall festival similar to the "Dutch Day" in the film.

Edgar E. Gore's name is a pun referring both to Ygor, the hunchback assistant to Dr. Wolfram Frankenstein in Son of Frankenstein (1939), and to Gothic writer/artist Edward Gorey. His voice is also a reference to Hungarian actor Peter Lorre.

This film contains many references and influences from horror cinema of many different sub-genres across motion picture history, from the 1920s to the 1980s. See Movie Connections.

The film was filmed at Three Mills Studios. In the movie, at the fair, there are casks of "Drie Molens" beer. "Drie Molens" in Dutch means "Three Mills."

Tim Burton created the Weird Girl ("Staring Girl") in his book "The Melancholy Death of Oyster Boy & Other Stories" (1997).

One of Tim Burton's famed director trademarks is absent from this movie. Most Burton movie opening credits "follow" a person or object, this one's credits do not.

Persephone the poodle is named after the Greek nature goddess who was kidnapped by and forcibly married to Hades, god of the underworld and lord of the dead.

This is the first animated Tim Burton film to not be a musical.

This film is loosely based on the live action short film Frankenweenie (1984), written and directed by Tim Burton in 1984.

The clock in Victor's attic workshop carries the brand of Miles Paulley, art department assistant coordinator.

The character Mrs. Frankenstein (Victor's mother) resembles Barbara Bain, once married to Martin Landau, who provides the voice for the character Mr. Rzykruski.

This movie's soundtrack features "This Song is a Curse," the first solo project of Frank Iero, guitarist of My Chemical Romance.

The second animated film to be directed by Tim Burton and the first to be solely directed by him.

John August was approached to do a rewrite of the Frankenweenie (1984) script in 2006.

Winona Ryder appears in this film, while Sofia Coppola appeared in the original short, Frankenweenie (1984). Coppola replaced Ryder when she withdrew from The Godfather: Part III (1990).

The town's name New Holland was once the name of the island continent of Australia from the mid 1600s till the 1800s. It was named by the Dutch explorer Abel Tasman after whom the Australian island state Tasmania was named.

Tim Burton: [dogs] Sparky the dog gets horribly damaged and altered.

When Victor goes to the pet cemetery to dig up Sparky, he throws some dirt onto a grave saying 'Goodbye Kitty' which resembles the 'Hello Kitty' logo.

The turtle Shelley's monster form is based on Gamera: The Giant Monster (1965) and its sequels.