Gru (voice)
Lucy (voice)
Eduardo Pérez / El Macho (voice)
Margo (voice)
Dr. Nefario (voice)
Floyd Eagle-san (voice)
Silas Ramsbottom (voice)
Agnes (voice)
Edith (voice)
Antonio Pérez (voice)
Jillian (voice)
Shannon (voice)
Kevin the Minion / Bob the Minion / Stuart the Minion / Additional Minions / Evil Minions (voice)
Additional Minions / Evil Minions / Italian Waiter (voice)
Arctic Lab Guards (voice)
Flight Attendant (voice)
Additional Voices (voice)
Additional Voices (voice) (as Claira Titman)
Additional Voices (voice) (as James Kevin Ward)