The script took nine years to make.

Despite widely published reports that Angelina Jolie was originally cast as the lead, but dropped out, Luc Besson has since denied them and admitted that he only met with a couple of actresses to discuss playing the lead role. He says when he saw how strongly Scarlett Johansson reacted to the story, she immediately landed the part.

In an interview on March 8, 2014, (evening news on France 2), Luc Besson said that this project took ten years to become a reality. Also, he admitted that he knew that some scientific assumptions were erroneous, e.g. that humans use only ten percent of their brains. Nonetheless, he said that "(such an assumption) would be a great start for a sci-fi movie".

The human-looking ape seen in some scenes is Australopithecus afarensis, who represents the "missing link" between apes and humans, nicknamed "Lucy" after John Lennon's song "Lucy in the Sky with Diamonds." She lived 3.2 million years ago and is the earliest known hominid to walk upright like humans.

Luc Besson once called this film as "one part Léon: The Professional (1994), one part Inception (2010) and one part 2001: A Space Odyssey (1968)". Many shots in the film were mirrored after these three films.

Although the scenes in Asia take place in Taiwan, Mr. Jang and his henchmen speak Korean.

When Lucy is in Times Square, over her right shoulder is a billboard for The Family (2013), the prior Luc Besson film.

In 2014, Lucy was the most successful French movie outside of France for the past twenty years, when UniFrance started creating statistics about French movies. With 463 million dollars, 52.1 million admissions outside of France, and 5.2 million admissions in France, Lucy was more successful than Taken 2 (2012), The Fifth Element (1997), and The Intouchables (2011).

A young tattoo artist is seen sporting a Mexican Lady of Guadalupe/Holy Death tattoo on her back. This mystery girl is also seen being tattooed in the movie 3 Days to Kill (2014). Both films were written by Luc Besson. The tattooed model is not credited in any of the films.

This is the first R-rated film to be partially shot with IMAX cameras.

The idea that humans only use 10% of their brains is a widely-circulated myth that has been debunked by neurological science many times over. (Humans typically use about 10% of their brains at a time.)

Milla Jovovich was rumored to play Lucy, but declined due to pregnancy.

During the car chase to the outdoor market, an old man reads a newspaper with an ad for Pacific Rim (2013) on the top of the front page.

Lucy's full name is "Lucy Miller" from her passport.

Lucy's birthday is March 10, 1988.

In 1974, Donald C. Johanson discovered a 3.2 million year old skeleton in Ethiopia. He called it Australopithecus Afarensis, later nicknamed "Lucy". In the movie, the lead character Lucy is played by Scarlett Johansson. (Despite the similarities of their surnames, the two are not related.)

In the trailer, a close up of Lucy's eye briefly shows a reptilian eye.

The first scene where Scarlett Johanson appears, is the Regent Taipei Hotel.

At the beginning of the movie, when Lucy is being approached by the South Korean thugs, the scene changes several times to footage of a cheetah chasing down and catching an gazelle. Lucy is wearing a cheetah-print jacket at the time.

Luc Besson: a doctor in the Paris hospital with the other three drug mules arrested, later among those shot by Mr. Jang's gang.

When prehistoric Lucy reaches her hand out to touch the other Lucy's hand, their hand/finger positions mirror the hand positions of Adam reaching out to touch the Hand of God in, "The Creation", by Michelangelo as painted on the ceiling of the Sistine Chapel, in Rome. Metaphorically, Lucy at one hundred percent brain capacity, could be seen as God, while prehistoric Lucy could be seen as an Adam figure.

At the end of the movie, when Morgan Freeman is holding the USB key that Lucy has created for him, Orion's Belt in the constellation of Orion is clearly seen in the surface of the key as it provides a glimpse of a glimmering out space.

The third known science fiction subject involving time traveling back to the birth of the universe. The only others are Altered States (1980) and the Star Trek: Voyager (1995) episode Death Wish. In Altered States, William Hurt's character has a very similar experience as Lucy.

When Lucy is chained and locked up in the room by the Korean drug cartel, she is hit in the stomach by the Korean guy. Drugs start leaking into her stomach and her body, and she starts shaking and going up the wall and ceiling. When she is leaning against the wall and crawling up it, the way the gravity affects the chains and her hair shows that she is actually lying on the floor.

Scarlett Johansson and Morgan Freeman have both appeared in comic book films. Johansson has played Natasha Romanoff/Black Widow in Iron Man 2 (2010), The Avengers (2012), and Captain America: The Winter Soldier (2014). Freeman played Lucius Fox in The Dark Knight Trilogy (2005-2012).