Brian
Alice / Soldier
Tripp / Agent RS-126 / Mr. President / Sexy British Narrator / Soldier
Moonshine / Dr. Wallace / Agent RS-126
Dr. Vonmurderahl
Agent Q-504 / Soldier / Shaman / Ashumpere
Agent CX-88 / Awkward Commuter
Tim (from work)
The Intelligence / Ice King
Atun-Shei
Atun-Shei's Skeleton (as 'Susan')
Detective Murphy
Detective Johnson
Surgeon
Voice of Surgeon (voice)
Nurse
Voice of Nurse (voice)
Soldier
Shaman
Shepherd
Shepherd (as Ian 'The Legend' Leisy)
Self (archive footage)
Brian's Cat