The track was released in January 2003 as the album's lead single and peaked at number one on the Billboard Hot 100, becoming 50 Cent's first number one single on that chart.
At the 46th Grammy Awards, it was nominated for Best Male Rap Solo Performance and Best Rap Song.
The song's music video won Best Rap Video and Best New Artist at the 2003 MTV Video Music Awards.
In 2009, the single was listed at number 24 in Billboard's Hot 100 Songs of the Decade.
In 2010, it was ranked 448th in Rolling Stone's 500 Greatest Songs of All Time list.
It is considered by many to be one of 50 Cent's best songs, if not his best song, by many fans and is one of his many well known and recognizable songs, even in rap in general.
"In da Club" was the first of seven tracks 50 Cent recorded in five days with Dr. Dre.
The Source called the song a "guaranteed party starter" with its "blaring horns, funky organs, guitar riffs and sparse hand claps".
The BBC wrote that the song is "a spectacular party anthem" that "highlights 50 Cent's ability to twist his words effortlessly".
Entertainment Weekly noted that 50 Cent "boasts unashamedly of his career objectives and newly flush bank account" with lyrics such as "I'm feelin' focus, man, my money on my mind/Got a mil out the deal and I'm still on the grind."
Rolling Stone wrote that the song sports "a spare yet irresistible synth hook augmented by a tongue-twisting refrain".
The Guardian called the track "irresistible" due to its "sparse orchestral samples and snaking chorus".
Pitchfork Media said, "the bounce on 'In da Club' is straight-up irresistible, Dre at both his minimalist best and most deceptively infectious."
Splendid magazine called the song an "insanely catchy" single with its "stanky, horn-addled thump".
The track was listed at number ten on Blender magazine's "The 500 Greatest Songs Since You Were Born".
It peaked at #1 for 9 weeks on the Billboard Hot 100 and remained on the chart for 30 weeks.
The track reached #1 on the Top 40 Tracks, Hot R&B/Hip-Hop Songs, and Hot Rap Tracks charts.
In March 2003, it broke a Billboard record as the "most listened-to" song in radio history within a week.
Nominated for Best Male Rap Solo Performance and Best Rap Song at the 2004 Grammy Awards, it lost to Eminem's "Lose Yourself".
Across Europe, it reached number one in Denmark, Germany, Ireland, Switzerland, Austria, Belgium, Finland, Greece, Norway, Sweden, and the Netherlands, and a number three in United Kingdom.
In Australia, the single peaked at number one, was certified two times Platinum by the Australian Recording Industry Association, and on the 2003 year-end chart, it was listed at number five.
On January 27, 2003, the video debuted on MTV's Total Request Live at number nine and stayed on the chart for fifty days.
At the 2003 MTV Video Music Awards, the video was awarded Best Rap Video and Best New Artist and was nominated for Video of the Year, Best Male Video, and Viewer's Choice.
In January 2006, 50 Cent was sued for copyright infringement by former 2 Live Crew manager Joseph Weinberger, who owns the rights to the rap group's catalog. He claimed that 50 Cent plagiarized the lines "it's your birthday" from the eponymous second track of former 2 Live Crew frontman Luther Campbell's 1994 album Freak for Life 6996. The lawsuit was dismissed by U.S. District Judge Paul Huck, who ruled that the phrase was a "common, unoriginal and noncopyrightable element of the song".