Out of all of the actors and actresses who have appeared, only David Caruso (Horatio Caine) and Emily Procter (Calleigh Duquesne) have been seen in every episode from the beginning.

Believing that CSI copycat shows were inevitable, CSI producers and CBS agreed to create this spin-off series, in the hopes of being the first to copy the original CSI series.

David Caruso is right-handed, but left eye dominant, evident when he is holding or shooting his pistol.

While the majority of the techniques and technologies used in the CSI shows are accurate and true to reality, the writers and crew readily admit that they "time cheat". Tests that take a few seconds on the show often take several days or weeks in real life.

Food items are frequently used to simulate injuries to corpses (for example, roast chicken skin is used to simulate burns.)

In season nine, Emily Procter (Calleigh Duquesne) was pregnant in real life. As a result, they had her behind desks, filmed her from the back and sides, and did close-ups of her.

Kim Delaney's exit from the show, early in the first season, was due to a lack of on-screen chemistry between her and David Caruso. The show runners used a so-called "trap-door" plot line, in which her character's letter to Horatio implied that she had returned to work too soon after having witnessed her husband's murder, and was still grieving the loss, therefore was unable to handle the rigors of the job anymore as head of the crime lab and was passing the torch to Horatio.

Sela Ward was offered the role of Megan Donner, but turned it down. She later went on to star in CSI: NY (2004).

CSI: Miami: Darkroom (2006) was personal for Eva LaRue (Natalia Boa Vista), because her sister Nika LaRue was photographed by serial killer William Richard Bradford. Nika was offered the role of Anya Boa Vista, but turned it down, due to the fact that it brought up very painful memories.

In real life, the Miami-Dade Police Department's crime scene processing unit is called the "Crime Scene Investigations Bureau (CSIB)", and unlike the television show, MDPD's CSIB technicians do not conduct laboratory testing. Miami-Dade Police Department has a separate Bureau that operates the Miami-Dade Crime Lab. Also, the CSIBs are not detectives, and most present day applicants are surprised to discover that the CSIBs do not perform most of the tasks depicted on the series. For example, they do not interview suspects, they do not write or execute search warrants, and they do not make arrests. In real life, they are directed around the scenes by the detectives and supervisors, not the other way around. Detectives are commissioned police officers (sworn personnel). CSIBs are civilian personnel, not sworn, and do not have the same arrest powers as police officers. However, they are very skilled technicians, and are a component of the police response to crime.

During the opening credits, the actors' names morph out of equations: - 4y - 1 = 3b(Nh) becomes David Caruso - 3a1 - X = (A9Xy) becomes Emily Procter - 3b + N = 7bn1(6A) becomes Adam Rodriguez - 2b + 4a = (7h)3XyNh becomes Khandi Alexander - A1b + B2c = R4 becomes 'Kim Delaney' - (7b) = 6m + (3h) becomes Jonathan Togo - (3h) + (7b) = 6 becomes Rory Cochrane.

The character of Lieutenant Horatio Caine is loosely based upon LAPD bomb squad technician Detective John Haynes.

Don Johnson was considered for Horatio Caine.

While all the fly-over shots, skylines and cityscapes really are of Miami, Florida, any beach scenes during the series were filmed at Manhattan Beach, a suburb of Los Angeles.

Horatio was named after the Victorian era "rags to riches" novelist Horatio Alger.

When some of the CSIs makes a test of DNA, fingerprints, or something similar, the percentage shown in the screen of the computer to sign to the guilty always is 99.32 percent.

CSI: Crime Scene Investigation (2000) star William Petersen was offered the chance to produce this show, but declined.

According to a March 2003 article in TV Guide Magazine, producer Anthony E. Zuiker refused to cast David Caruso because of his reputation for being difficult. However, after seeing Caruso's performance in Proof of Life (2000), CBS President Leslie Moonves convinced him to reconsider.

The phone number on the MDPD squad cars - (305) 4-POLICE - is the real non-emergency number to the Miami-Dade Police Department.

Much like the other CSI series, many of the cases portrayed are based on real-life crimes. The writers make certain changes, such as names, location, and other details for obvious reasons, but some details, such as manner of death and how the crime was committed closely echo the real crime.

Real-life prosecutors have complained about something known as the "CSI Effect", where juries have unrealistic expectations about forensic science, either expecting copious amounts of forensic evidence, in even routine cases, or expecting an unrealistic level of accuracy and specificity from the tests presented.

In every scene that includes a shot of a street, sidewalk, et cetera, the street, sidewalk, et cetera will be wet, giving an appearance that it just rained. It could be to set the mood, and imply the humidity of Florida (the over exposed exterior shots are to give the visual feel of the heat), common practice to ensure consistency between shots if a rain storm happens to pass through, a way to remove any trace of a reset scene (for example, wash fake blood away) or the director just likes the aesthetic.

The series was cancelled on Sunday, May 13, 2012.

Although some beach scenes may have been shot outside of Southeast Florida, several have been shot in Miami-Dade, Broward, and Palm Beach counties.

Virtually all of the outdoors scenes are shot in Long Beach, California. Most are filmed in the Belmont Shore and Naples neighborhoods. The rest are downtown.

To date, is the only CSI series not to have an Academy Award nominee as the leading cast member, or as a regular cast member.

The building used for the CSI Headquarters is actually the FAA Credit Union In Hawthorne, California. The building was also used as the Newport Group's offices in the show The O.C. (2003).

At the conclusion of each case, the culprits almost always confess their guilt to investigators, that would most assuredly not be the people interviewing them, this helps to wrap up the case in a Scooby Doo-like manner for the general viewing public.

Although the City of Miami counts thirty-five percent of its population as Cuban, Adam Rodriguez is the only cast member to be of Cuban descent (he is one fourth Cuban).

Two frequent characters, Calleigh Duquesne and Maxine Valera, have names that closely resemble those of two installations in the Skylark SciFi series by Edward E. Smith.

The design of the autopsy theater was based on a misremembering of a set from The Andromeda Strain (1971).

In "CSI: Miami" (2002) {Backfire (#8.20)}, Wolfe and Walter are shifting through debris from the fire, looking for the point of origin. The victim's ghost enters the room, and Walter asks if anyone else felt a breeze. Wolfe quips "you've been watching too many Rob Zombie movies." This is a nod to the musician, who directed CSI: Miami: L.A. (2010).

Wes Ramsey, who plays Dave Benton from the episode "CSI: Miami" (2002) {Divorce Party (#7.17)}, had a previous role in "CSI: Miami" (2002) {Spring Break (#1.21)} as a sexual predator who stalked girls to abuse them.

The final season of CSI: Miami is also the shortest of the series, lasting only nineteen episodes.

On the flip side of the CSI effect, kidnap and rape victims are now known to leave their own DNA behind at crime scenes to give police forensic evidence to find. An example of this is how British serial sex attacker Jonathan Haynes was eventually caught. Haynes, who had avoided capture by forcing his victims to destroy forensic evidence was finally apprehended after the CSI effect was used against him when one of his victims ensured her DNA could be traced back to her attacker by spitting in his car and pulling out strands of her own hair. Her inspiration for this act? CSI.

Lieutenant Caine nicknames Ryan Wolfe as Mr. Wolfe, being the only one of the CSI with this kind of formal treatment. Phonetically, "Mr. Wolfe" sounds exactly as Harvey Keitel's character in Pulp Fiction (1994), who was named The Wolf or "Mr. Wolf".

Real life police and prosecutors have noticed due to the CSI effect real-life criminals have started covering their tracks and are destroying potential evidence against them before leaving crime scenes. One example of this is when double-murderer Jermaine McKinney broke into a house and killed a mother and daughter, he then proceeded to wash blood away with bleach, burn his clothing to destroy evidence, blanket his getaway car to avoid blood transfer, remove his cigarette butts from the scene and attempt to dispose of the murder weapon. McKinney was a big fan of CSI, and this type of calculated behavior is no isolated incident as some rapists have now begun forcing their victims to shower after an attack in order to wash away any forensic evidence. "[These shows] are actually educating potential killers even more," says Head of Los Angeles Homicide Division Captain Ray Peavy. "Sometimes I believe it may even encourage them when they see how simple it is to get away with on television." Peavy isn't alone in his thinking. "[Criminals] do clean up, and they tend to clean up much more carefully now," said Linda Johnson, a crime lab director at Jefferson County, Alabama. "A lot of them know they can use bleach and different detergents to mask our ability to take blood."

The "Universal Channel" (UK and Ireland), for their early morning double bill showing in 2016, ran a slightly edited version of the series, to remove the more graphic visuals. Also, other revisions within each episode included some dialogue cut, or trimmed, and certain crime scene/autopsy table/computer screen visuals cropped and/or blurred. Entire episodes were also skipped over, most likely due to the relevant episode story themes and visual content unsuitable for broadcast at that time of day. However, when the CBSdrama channel (UK &Ireland) started re-running the series in 2019, the series was seemingly unedited. Example - in series 1 episode 3, on Universal, the torso shots were edited out/cropped out of frame, and blurred on the autopsy tv screen, but CBS didn't, so in effect there's alternate broadcast edits.