Batman / Bruce Wayne (voice)
Robin / Tim Drake (voice)
Nightwing / Dick Grayson (voice)
Alfred Pennyworth / TV Host (voice)
Batgirl / Barbara Gordon (voice) (as Tara Charendoff)
Det. Harvey Bullock (voice)
Commissioner James Gordon (voice)
Det. Renee Montoya (voice)
Jack Ryder / The Scarecrow / Dr. Jonathan Crane / Police Officer (voice) (as Jeff Glen Bennett)
Mayor Hamilton Hill (voice)
The Mad Hatter / Jervis Tetch (voice)
Lawyer (voice)
Harley Quinn / Dr. Harleen Quinzel (voice)
Bane (voice)