Sue Sylvester Muppet (voice)
Quinn Fabray Muppet (voice)
Kurt Hummel Muppet / Morty the Musical Muse / Nose McDonald (voice)
Abby Cadabby / Brittany Pierce Muppet / J / Velvet (voice)
Elmo (voice)
Rachel Berry Muppet (voice)
Luis
Self
Grover / G (voice)
Artie Abrams Muppet (voice)
Chris (as Christopher Knowings)
Maria
Murray Monster / Blogg / Horatio the Elephant / Mr. Gue (voice)
Bob
Alan
Gordon
Rosita / Ovejita / Queen of Nacho Picchu (voice)
Gonnigan (voice)
Oscar the Grouch (voice)
Mrs. Sparklenose (voice)
Rhombus of Recipes (voice)
Leela